| icon | info | gamerscore |
|---|---|---|
| An Old Friend Returns Escape the hideout. | 100 | |
| The Vault Complete DNA Sequence 14. | 30 | |
| Victory lies in preparation Get all Hidden Blades, Item Pouches and Armor upgrades for Ezio. | 10 | |
| The Prophet Complete DNA Sequence 11. | 30 | |
| Bianca's Man Complete DNA Sequence 10. | 30 | |
| Podestà of Monteriggioni Reach 80% of your stronghold's total value. | 30 | |
| Masquerade Complete DNA Sequence 9. | 30 | |
| Venetian Gladiator Discover the Assassin's Tomb inside Santa Maria della Visitazione. | 20 | |
| The Impenetrable Palazzo Complete DNA Sequence 8. | 30 | |
| Fly Swatter Kick a Guard while using the Flying Machine. | 5 | |
| Hallowed be thy name Discover the Assassin's Tomb inside the Basilica di San Marco. | 20 | |
| The Merchant of Venice Complete DNA Sequence 7. | 30 | |
| Bleeding Effect Complete training and reenter the Animus. | 30 | |
| An Unexpected Journey Complete DNA Sequence 6. | 30 | |
| Prison Escape Discover the Assassin's Tomb inside the Rocca di Ravaldino fortress. | 20 | |
| Assassin For Hire Complete your first assassination mission for Lorenzo Il Magnifico. | 10 | |
| The Conspirators Complete DNA Sequence 5. | 30 | |
| Street Cleaner Hide 5 dead bodies in a Bale of Hay. | 10 | |
| I can see your house from here! Discover the Assassin's Tomb inside the Torre Grossa. | 20 | |
| Choir Boy Discover the Assassin's Tomb inside Santa Maria del Fiore (The Duomo). | 20 | |
| High Dive Perform a Leap of Faith from the Top of Florence's Giotto's Campanile. | 10 | |
| Bloody Sunday Complete DNA Sequence 4. | 30 | |
| Mailman Intercept a Borgia Courrier. | 10 | |
| Undertaker Discover the Assassin's Tomb inside the catacombs under Santa Maria Novella. | 20 | |
| I like the view Synchronize 10 View Points. | 10 | |
| Handy Man Upgrade a building in the Stronghold. | 10 | |
| Exit the Son Complete DNA Sequence 3. | 30 | |
| Myth Maker Find the 8 statuettes in Monteriggioni. | 5 | |
| Vengeance Complete DNA Sequence 2. | 30 | |
| Lightning Strike Sprint for 100 meters. | 10 | |
| A Piece of the Puzzle Unlock a piece of Subject 16's video. | 10 | |
| Tip of the Iceberg Use your Eagle Vision to scan a Glyph in the environment. | 10 | |
| The Pain of Betrayal Complete DNA Sequence 1. | 30 | |
| Macho Man Defend a woman's honor. | 10 | |
| Welcome to the Animus 2.0 Enter the Animus 2.0. | 20 | |
| Arrivederci Abstergo Break out of Abstergo. | 20 | |
| The Birth of an Assassin Be reborn as Ezio Auditore Da Firenze. | 20 | |
| Vitruvian Man Unlock all 20 pieces of Subject 16's video. | 20 | |
| Doctor Perform an Air Assassination on a Poisoned NPC. | 20 | |
| Kleptomaniac Pickpocket 1000 Florins. | 10 | |
| Perfect Harmony Tint your clothes with those colors: Wetland Ebony and Wetland Ivory. | 10 | |
| Art Connoisseur Buy a Painting from Florence and Venice. | 10 | |
| Man of the People Toss more than 300 florins on the ground. | 10 | |
| Steal Home Win a race against thieves! | 10 | |
| Red Light Addict Spend 5000 florins on Courtesans. | 10 | |
| No-hitter Kill 10 enemies while remaining in conflict without being hit. | 20 | |
| Show your Colors Wear the Auditore cape in each city. | 10 | |
| In Memory of Petruccio Collect all the Feathers. | 30 | |
| Messer Sandman Stun 4 guards at once by throwing sand in their face. | 10 | |
| Sweeper Sweep 5 guards at once by using a Long Weapon. | 10 |
