| icon | info | gamerscore |
|---|---|---|
| A Knife to the Heart Secure the Apple of Eden. | 50 | |
| Requiescat In Pace Complete DNA Sequence 9. | 20 | |
| Vittoria Agli Assassini Complete DNA Sequence 8. | 20 | |
| Show Off Complete 10 Guild Challenges. | 20 | |
| Kaboom! Destroy the Tank. | 20 | |
| Boom! Destroy the Bomber. | 20 | |
| Plumber Discover the Shrine in the Cloaca Maxima. | 20 | |
| Amen Discover the Shrine in the Basilica di San Pietro. | 20 | |
| Tower Offense Burn All Borgia Towers. | 20 | |
| Welcome to the Brotherhood Train a recruit up to the Rank of Assassin. | 20 | |
| Bloody Sunday Complete DNA Sequence 7. | 20 | |
| Spring Cleaning Kill a guard with a broom. | 10 | |
| One-Man Wrecking Crew Discover the Shrine in Palazzo Laterano. | 20 | |
| Déjà Vu Replay a Memory. | 20 | |
| Forget Paris Complete DNA Sequence 6. | 20 | |
| Splash! Destroy the Naval Cannon. | 20 | |
| Fundraiser Complete DNA Sequence 5. | 20 | |
| Dust to Dust Find 1 Artifact in 2012. | 20 | |
| Mailer Daemon Access your email in 2012. | 20 | |
| Principessa in Another Castello Complete DNA Sequence 4. | 20 | |
| Bang! Destroy the Machine Gun. | 20 | |
| Gladiator Discover the Shrine in Il Colosseo. | 20 | |
| Home Improvement Renovate 5 buildings in the Antico district. | 20 | |
| Brotherhood Recruit 3 Assassins. | 20 | |
| Serial Killer Perform an Execution Streak of 10 kills. | 20 | |
| Fixer-Upper Complete DNA Sequence 3. | 20 | |
| Perfect Recall Achieve 100% Synchronization in any Sequence other than Sequence 1. | 30 | |
| Undertaker 2.0 Discover the Shrine in the Catacombe di Roma. | 20 | |
| Rome in Ruins Complete DNA Sequence 2. | 20 | |
| Golden Boy Discover the Shrine in the Terme di Traiano. | 20 | |
| Sanctuary! Sanctuary! Find a secure place to hide and re-enter the Animus. | 20 | |
| Battle Wounds Complete DNA Sequence 1. | 20 | |
| Technical Difficulties Attempt to access DNA Sequence 9 for the first time. | 10 | |
| Strong Closer Take the lead 10 seconds before the end of a session and go on to win (Multiplayer Only). | 20 | |
| Fast Learner Kill your target and escape your pursuer in less than 10 seconds (Multiplayer Only). | 25 | |
| Synchronization Established Complete an entire Session with at least one kill (Multiplayer Only). | 10 | |
| Ahead of the Curve Perform a double or triple escape (Multiplayer Only). | 20 | |
| Job skills During Open Conflict, kill your target and escape (Multiplayer Only). | 20 | |
| Your Wish is Granted Throw money in a well. | 10 | |
| Role Model Get all the Co-op bonuses in 1 session (Multiplayer Only). | 20 | |
| Airstrike Kill 10 guards with a single arrow storm. | 20 | |
| Clowning Around Beat up the 5 thirsty harlequins. | 30 | |
| il Principe Receive 100% synch in ACB and full synch in all missions of the Da Vinci Disappearance. | 100 | |
| Overachiever Score 750 points or more on a single kill (Multiplayer Only). | 20 | |
| Easy Come, Easy Go Pay 500 florins to an orator, then pickpocket him afterward. | 5 | |
| Special Delivery Double Assassinate from a parachute. | 20 | |
| Strong-Arm Throw a Long Weapon, Heavy Weapon, and Smoke bomb more than 10 m at a guard. | 10 | |
| Capture the Flag Remove All Borgia Flags in Rome. | 30 | |
| High Roller Win 10000 florins playing Hazard. | 20 | |
| Perfectionist Earn 3 gold medals in the Animus Virtual Training Program. | 20 | |
| GPS Complete the Da Vinci Disappearance. | 20 | |
| Grand Theft Dressage Steal 5 horses from their riders, while remaining on horseback. | 20 | |
| .. .- -- .- .-.. .. ...- . Solve all of Subject 16’s puzzles. | 20 | |
| In Memoriam Collect All Feathers. | 20 | |
| Going Up Kill a guard with the bag that drops from a lift. | 5 | |
| The Gloves Come Off Win the highest bet at the Fights. | 10 | |
| Fly Like an Eagle Jump with the parachute off the top of Castel Sant'Angelo. | 10 | |
| Needle in a Haystack Kill your target while hidden in a hay bale (Multiplayer Only). | 5 |
